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150                                      Frutas del trópico







                    Internal CO  concentration (substomatal CO  concentration; internal partial pressure of
                                 2                                  2
               CO ) is the amount of CO  in the small stomatal cavity waiting to be fixed during photosynthe-
                   2                       2
               sis. By plotting net CO  assimilation over Ci (called an A/Ci curve) it can be determined if the
                                       2
               limitation to carbon fixation is due to stomatal or biochemical factors (Farquhar and Sharkey,

               1982). The initial linear portion of the regression line slope represents the non-stomatal limi-

               tation to photosynthesis. For example, Figure 3 shows A/Ci curves for mango trees growing

               in the field where there was no root restriction and in pots where there was root restrictions

               that resulted in an inhibition of photosynthesis.



                   Figura. 3. A/Ci curves of ‘Kensington’ mango plants in containers with and without root restriction
                                                   (from Schaffer et al., 1997).






                                       40
                                     A (µmol CO 2   m -2  s -1 )   30




                                       20


                                       10
                                                             Field-grown (non-root restricted)

                                        0                    Container-grown (root restricted)
                                         0    200   400   600   800  1000 1200 1400 1600
                                                                        -1
                                                      Ci (mmol CO  mol )
                                                                   2


                    There was no difference in the initial slope of the A/Ci curve between root-restricted and

               non-root-restricted plants, indicating that the reductions in net CO  assimilation as a result of
                                                                                      2
               root restriction was not related to biochemical causes such as a reduction in Rudp-carboxylase/








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